Re-using unloaded agarose gel
Posted 24 May 2010 - 03:57 AM
I am wondering if i can use unloaded wells in 2.5% agarose in another run given that the gel has been used only in one run and kept soaked in TAE at room temp for 8hrs. would it be okey if the gel has been used again....?
Posted 24 May 2010 - 05:41 AM
Also I run the gel the same day so I don't have experience with a prolonged storage at RT. If I want to use one gel for two times without compromising quality, I cut it before run and store the unused half in running buffer in a fridge.
Basically, if you value your samples you should make a new gel. I'm only doing it when I just need to check someting easily reproducible.
I never trust anything that can't be doubted.
Posted 24 May 2010 - 06:35 AM
Posted 24 May 2010 - 07:22 AM
Posted 25 May 2010 - 01:29 PM
Posted 26 May 2010 - 02:06 AM
This is what I do too, i remelt in microwave, re-add ethidium bromide and recast them.
Used up to 20 times and don't have much issue...it just looks dirty.
After all I use it to see whether there is band or no band so this will not be a problem for me.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 26 May 2010 - 05:45 AM