antibiotics for preventation of mycoplasma contamination
Posted 23 May 2010 - 02:21 AM
we usally use pen/strep in cell culture but recently we had some mycoplasma cintamination. which antibiotic is recommanded for preventing mycoplasma contamination in cell culture media?
Posted 23 May 2010 - 04:14 PM
In your routine cell culture you should not be using antibiotics at all as they mask cryptic contaminations that will be affecting your results.
Posted 27 May 2010 - 05:16 PM
Posted 30 May 2010 - 04:24 PM
Posted 30 May 2010 - 04:52 PM
very few things work against Mycoplasma. I second the "Into the bin" proposition.
The few agents that do work on mycoplasma, work slowly and are expensive. Furthermore they place a selection pressure on your cells that can make permanent changes to cellular phenotype
Mycoplasma Removal Agent (MRA), (MP Biomedicals)