DNA isolation from dark hair
Posted 22 May 2010 - 06:53 AM
I isolate DNA from white animal hair (with NaOH and HCl+Tris) and it works well. But It doesnt work with dark hair and I need to understand why. Could anybody help me? Is pigment in dark hair the reason or what? Thank u.
Posted 23 May 2010 - 04:07 PM
Posted 24 May 2010 - 12:49 PM
Leopold Eckhart,* Ju¨ rgen Bach,* Jozef Ban,*, and Erwin Tschachler*,,1
Melanin Binds Reversibly to Thermostable DNA
Polymerase and Inhibits Its Activity
Biochemical and Biophysical Research Communications 271, 726730 (2000)
Edited by Maddie, 24 May 2010 - 12:52 PM.
Posted 26 May 2010 - 06:04 AM
Posted 26 May 2010 - 11:06 AM
It's very hard not to lose any hair on a crime scene, especially if there is a fight.
When the hair is old, then it gets more difficult and you'll need several cm.