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Double transfection of siRNA


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#1 srm mrs

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Posted 21 May 2010 - 09:51 PM

Hi!

Is it possible to transfect cells simultaneously with two or more than two siRNAs? If yes then how do i optimize the concentrations required? How do i proceed? Plz help!

#2 pcrman

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Posted 22 May 2010 - 09:44 AM

It is possible, but be cautious that when you throw in two siRNAs into a cell, they may not function equally as expected because Ago2 protein has preference over certain thermodynamically structured siRNAs. So you need proper controls,eg, combination with a control siRNA. You should also check knockdown efficiency of each siRNA used alone or in combination.

#3 Born free

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Posted 24 May 2010 - 12:35 AM

It is possible, but be cautious that when you throw in two siRNAs into a cell, they may not function equally as expected because Ago2 protein has preference over certain thermodynamically structured siRNAs. So you need proper controls,eg, combination with a control siRNA. You should also check knockdown efficiency of each siRNA used alone or in combination.

Hi,

Thax for the reply.
So, hw do i optimize the simultaneous transfection?




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