Log 10 fold serial dilutions
Posted 21 May 2010 - 08:54 PM
Im performing an absolute quantification assay using real time PCR, and for this i am constructing a standard curve with linearized plasmid DNA. Does any one know how to prepare log 10 fold dilutions of the template DNA.?
Posted 21 May 2010 - 08:58 PM
Posted 21 May 2010 - 11:26 PM
Exactly. And vortext vigorously between steps.
BTW: start with 10e8 copies of your plasmid