do i have to count my cells for a few intervals, to get an impression when they are in the log phase...?
how this should be handled? do i have to trypsinate my cells to get them suspended??
is it that important to know the exact initiation of the log phase - the log phase lasts for hours or days - or no???
how this is normally done?
thx for any suggestions
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bob1
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Thelymitra pulchella
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Excellent
Posted 24 May 2010 - 05:17 PM
To do this you need to do a growth assay of some sort. Lifting and counting wells at defined time-points of a plate seeded at a starting density is one way to do this. Other ways are MTT and/or DNA content assays, which can be done in 96 well plates.
