I want to express a mirna in the liver of zebrafish ？
How to build this kind of vector?
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how to expression mirna in a specific tissue ？
1 reply to this topic
Posted 21 May 2010 - 07:45 PM
You will need to clone the precursor miRNA sequence (the pre-miRNA sequence plus 100-200 bp flanking sequences on both sides) into a expression vector and the transcription of the miRNA in the vector should be driven by a tissue (liver) specific promoter.