Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

how to expression mirna in a specific tissue ?


  • Please log in to reply
1 reply to this topic

#1 luc

luc

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 21 May 2010 - 05:18 AM

I want to express a mirna in the liver of zebrafish ?
How to build this kind of vector?
thank you

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 21 May 2010 - 07:45 PM

You will need to clone the precursor miRNA sequence (the pre-miRNA sequence plus 100-200 bp flanking sequences on both sides) into a expression vector and the transcription of the miRNA in the vector should be driven by a tissue (liver) specific promoter.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.