Reviving a hybridoma from LN2
Posted 20 May 2010 - 07:27 AM
We're about to revive some frozen hybridoma cells and the procedure recommends co-culturing with normal mouse spleen cells until the hybridoma cells can proliferate on their own. I'm having trouble finding a source of normal mouse spleen cells.
1. Is it necessary to co-culture?
2. Sources of normal mouse spleen cells?
3. Alternatively I think we can just use conditioned medium but the source I found (Sigma) has it back ordered until July. Any other conditioned medium sources?
Posted 20 May 2010 - 09:57 AM
Posted 30 August 2010 - 06:48 AM