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Using Z-VAD-FMK in culture


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#1 positivecontrol

positivecontrol

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Posted 20 May 2010 - 04:42 AM

When I knock down a certain gene in cultured fibroblasts, they gradually undergo more apoptosis (by Annexin-V) than mock-knockdown fibroblasts, over a 5-10 day period.

I would like to test if this is caspase mediated apoptosis, using the pan-caspase inhibitor Z-VAD-FMK. Does anyone know how long this peptide lasts in culture, i.e. how often should I add it over a 5-10 day period?

Can't seem to find literature on this, but maybe someone here knows? Thanks!




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