Hey guys,
Someone put forward this question to me today:
If I have a primer (5'-3'), can it bind to a complementary DNA sequence (5'-3' also)?
Sort of like (example only, yeah)
5'-AGCTTGCTGAGTGC-3'
5'-AACGACT-3'
I guess. Is this scenario possible?
If it binds, can this pairing be amplified in PCR?
What do you guys think?
Chris
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#1
chrisbelle
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Posted 20 May 2010 - 12:24 AM
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Trof
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Posted 20 May 2010 - 03:57 AM
Binding is always in the oposite orientation of strands.
So like this in this case:
5'-AGCTTGCTGAGTGC-3'
3'-TCAGCAA-5'
That means it's not complementary except for some weak 2-3bp interactions.
Check it with IDT Oligo Analyser.
So like this in this case:
5'-AGCTTGCTGAGTGC-3'
3'-TCAGCAA-5'
That means it's not complementary except for some weak 2-3bp interactions.
Check it with IDT Oligo Analyser.
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