Can a primer bind to DNA in a 5'-3 parallel fashion?
Posted 20 May 2010 - 12:24 AM
Someone put forward this question to me today:
If I have a primer (5'-3'), can it bind to a complementary DNA sequence (5'-3' also)?
Sort of like (example only, yeah)
I guess. Is this scenario possible?
If it binds, can this pairing be amplified in PCR?
What do you guys think?
Posted 20 May 2010 - 03:57 AM
So like this in this case:
That means it's not complementary except for some weak 2-3bp interactions.
Check it with IDT Oligo Analyser.
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