Im having problems with my MCF-7 cells...
I seed the cells in a microplate and let them to atach for 24h. Then, I put my drugs to test and let more 24h.
When I wash the cells I notice that they detach from the well... The monolayer sometimes seems to fold.
I already worked with Vero cells and I didn't have this problem... And also I know (think) now that is not a error in the wash step.
What could be wrong? It could be the type of plate (I used Costar)? Should I use some substrate for the cells attachment?
Edited by Stone, 19 May 2010 - 06:09 PM.