2 replies to this topic

[Stone]

Hi!

Im having problems with my MCF-7 cells...

I seed the cells in a microplate and let them to atach for 24h. Then, I put my drugs to test and let more 24h.
When I wash the cells I notice that they detach from the well... The monolayer sometimes seems to fold.

I already worked with Vero cells and I didn't have this problem... And also I know (think) now that is not a error in the wash step.

What could be wrong? It could be the type of plate (I used Costar)? Should I use some substrate for the cells attachment?

Edited by Stone, 19 May 2010 - 06:09 PM.

[bob1]

Different cell lines behave differently.  MCF-7 are not particularly strongly attached to their substrate.  You could try gelatin (collagen) coating the wells.

[Stone]

bob1, on May 19 2010, 10:59 PM, said:

Different cell lines behave differently.  MCF-7 are not particularly strongly attached to their substrate.  You could try gelatin (collagen) coating the wells.

Thanks for the reply!

I was wondering why the MCF-7  looks well attached at the cell culture flask and not in the microplate..