PCR template concentration
Posted 19 May 2010 - 11:40 AM
I am running a QPCR and with sybr green and I'm not sure how much template to use. I am going to titrate my template but am not sure what to use for my high-end titration point. My template stock concentration is 100um. Any advice?
Posted 19 May 2010 - 12:27 PM
Posted 19 May 2010 - 02:54 PM
I'm sorry but I don't know what you mean....
Posted 19 May 2010 - 03:33 PM
Posted 20 May 2010 - 12:08 AM
What kind of template you have? Plasmid? cDNA? PCR product?
What is your template concentration? I don't think it is 100 uM. You were talking about primers probably.
Posted 20 May 2010 - 03:10 AM