Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

dye for practice brain injection


  • Please log in to reply
5 replies to this topic

#1 T. Hsu

T. Hsu

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 19 May 2010 - 11:23 AM

Hi,

I'm going to be injecting toxin into mouse brains using a stereotaxic device. But before I inject my precious experimental mice, I want to make sure that I have my coordinates right. So I want to inject a dye into the brain for my practice run, fix the animals, section the brains and see if I hit my targert correctly. Does anyone have any suggestion as to which dye to use? Thanks.

#2 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
7
Neutral

Posted 19 May 2010 - 11:41 AM

You could try Evan's blue, which is what we used for mouse foot pad injections.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 gfischer

gfischer

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 195 posts
9
Neutral

Posted 20 May 2010 - 08:41 AM

Pontamine Sky Blue is another good option. Fast Green could work as well.
Above all things, if kindness is your king,
then heaven will be yours, before you meet your end

#4 T. Hsu

T. Hsu

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 21 May 2010 - 11:53 AM

Pontamine Sky Blue is another good option. Fast Green could work as well.



Thanks!

I've heard of Evans blue and fast green, but not pontamine sky blue. Any suggestion on concentration?

#5 JSD

JSD

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 18 September 2010 - 03:54 PM

Pontamine Sky Blue is another good option. Fast Green could work as well.



Thanks!

I've heard of Evans blue and fast green, but not pontamine sky blue. Any suggestion on concentration?

India Ink at a dilution of 1:100 is another option. I've used it for practice injections, but in my case I did not fix tissue after injection, simply harvested the brain, made a coronal cut with a blade at site of injection and checked under dissection scope.

#6 scolix

scolix

    a student

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 314 posts
4
Neutral

Posted 11 November 2010 - 11:19 PM

any dye would work, even trypan blue.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.