Band appear and disapper in the negative control ??
Posted 19 May 2010 - 09:28 AM
I checked my gel to see if PCR was succesful about 10 minutes after loading. Bands were visible on both samples and negative control . It had not run long enought to be able to read the ladder. Placed the gel back in the box and ran an additional 25 minutes. Checked my PCR on the UV box again and my PCR bands and the ladder still present, but the band i saw on the negative control has disappeared (which is somthing good for me). Can anyone help me identify what could have happened? Thank you in advance.
Posted 17 June 2010 - 06:31 PM
Posted 21 June 2010 - 11:34 AM
Edited by Maddie, 21 June 2010 - 11:34 AM.