I can not get serum from mice blood samples (bleeding from retro orbital plexus). The clot donīt form properly . The same mice were bled some weeks ago with no problems. any clues on why this could happen? Thanks

Posted 19 May 2010 - 09:14 AM
Posted 21 May 2010 - 07:12 AM
Posted 21 May 2010 - 01:36 PM
Hi there,
How much blood are you getting? What kind of blood tubes are you using? How long are you waiting for the blood to clot? What speed are you centrifuging? I haven't used serum for my rodents as of yet just plasma, but in my vet clinic experience for dogs and cats, we used serum separator tubes (they have a red top or marble top usually), inverted about 5 times, let it sit about 10 minutes to clot, and then you centrifuge the tubes.
http://www.bd.com/va...uide_VS5229.pdf This site from BD goes over tubes and additives.
http://www.bd.com/va...mple_VS5177.pdf Procedure for BD serum tubes
Hope this helps. Let me know how it works out for you.
Stacy
Posted 24 May 2010 - 07:04 AM
Posted 26 May 2010 - 03:45 AM
Edited by Gerard, 26 May 2010 - 03:49 AM.
Posted 26 May 2010 - 04:38 AM
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