hi guys:
recently i have a experiment about detecting the ubiquitiantion protein in dendritic cell. but in my lab, there are nobody to do this. as a newer, i cannot do it by myself. can someone do a fever. thank you very much.
if there are some information about proteasome inhibitors, i will be lucky!
best wish!!
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#2
Dr Teeth
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Posted 19 May 2010 - 06:03 AM
yulang, on May 19 2010, 10:00 AM, said:
hi guys:
recently i have a experiment about detecting the ubiquitiantion protein in dendritic cell. but in my lab, there are nobody to do this. as a newer, i cannot do it by myself. can someone do a fever. thank you very much.
if there are some information about proteasome inhibitors, i will be lucky!
best wish!!
recently i have a experiment about detecting the ubiquitiantion protein in dendritic cell. but in my lab, there are nobody to do this. as a newer, i cannot do it by myself. can someone do a fever. thank you very much.
if there are some information about proteasome inhibitors, i will be lucky!
best wish!!
MG-132 is a commonly used 26S-proteasome inhibitor.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#3
mdfenko
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Posted 19 May 2010 - 06:54 AM
there are a few listed at this wikipedia page.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#4
Another Jake
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Posted 19 May 2010 - 08:00 AM
Lactacystin costs a lot more than MG132, but is also more effective and specific.
I once used an even better one called Velcade, but it was extraordinarily expensive.
I once used an even better one called Velcade, but it was extraordinarily expensive.
#5
yulang
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Posted 19 May 2010 - 08:36 PM
thanks for your replies! i am very appreciated. but the question is my cell line-dendritic cell. i found that some proteasome inhibitors(MG132) can influence the cell line (e.g:promote the cell appotosis). so i want to know if there are proteasome inhibitor having slight influence on dendritic cell.
and i will do CO-IP about ubiquitiate protein. does anybody have some protocol about that?
thank you very much.
and i will do CO-IP about ubiquitiate protein. does anybody have some protocol about that?
thank you very much.
