4 replies to this topic

[moljul]

what are classical inducers of apoptosis (e.g. staurosporine)?

hopefully get a few examples! don´t know which to purchase!!

thx in advance

[moljul]

moljul, on May 19 2010, 03:06 AM, said:

what are classical inducers of apoptosis (e.g. staurosporine)?

hopefully get a few examples! don´t know which to purchase!!

thx in advance

juhu, I found a pretty important site: http://www.promokine...tosis-inducers/

Edited by moljul, 19 May 2010 - 06:29 AM.

[than4]

To select a positive control you might need to let us know what your test samples are.

Some apoptosis inducers will induce the "death-receptor" pathway such as TRAIL or Fas ligand and some will induce the "mitochondrial" pathway such as etoposide, staurosporine etc.  You can also induce apoptosis in some cell lines by UV irradiation or serum starvation.

You might need to think about which pathway your test samples might induce and select an appropriate positive control that works in a similar way.

[moljul]

than4, on May 19 2010, 03:49 PM, said:

To select a positive control you might need to let us know what your test samples are.

Some apoptosis inducers will induce the "death-receptor" pathway such as TRAIL or Fas ligand and some will induce the "mitochondrial" pathway such as etoposide, staurosporine etc.  You can also induce apoptosis in some cell lines by UV irradiation or serum starvation.

You might need to think about which pathway your test samples might induce and select an appropriate positive control that works in a similar way.

thanks than4 for this recommendation. in the case of FACS (annexin V) staining it does no matter i think?
maybe it does matter, if i want to check for different activated caspases....eg -3 or -8!

Edited by moljul, 19 May 2010 - 10:34 PM.

[Inmost sun]

moljul, on May 19 2010, 12:06 PM, said:

what are classical inducers of apoptosis (e.g. staurosporine)?

hopefully get a few examples! don´t know which to purchase!!

thx in advance

we also use vinblastine