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Protocol for Blue Sepharose 6 Fast Flow purification

Started by Sharky, May 19 2010 02:39 AM

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#1 Sharky

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Posted 19 May 2010 - 02:39 AM

Hey guys,
             Does anyone have a decent protocol, especially for the buffers to use for Binding/Washing/Elution for the Blue Sepharose 6 Fast flow column from GE?

My protein has a pI= 6.19

#2 mdfenko

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Posted 19 May 2010 - 07:06 AM

have you checked the blue sepharose 6 ff handbook?

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