4 replies to this topic

[deep]

Can anybody tell me how to obtain micromolar concentration of any drug.
I want to test 2.5 and 5 micromoles of drug in cell culture. molecular weight of the drug is 264.32.

[than4]

deep, on May 19 2010, 01:13 AM, said:

Can anybody tell me how to obtain micromolar concentration of any drug.
I want to test 2.5 and 5 micromoles of drug in cell culture. molecular weight of the drug is 264.32.

I would made up a millimolar stock (say 1mM) and dilute appropriately to get micromolar concentrations in your media.

e.g. for a 1uM concentration in 1mL of media add 1uL of 1mM stock.

[mdfenko]

do you want concentration (micromolar) or amount (micromoles)?

based on the formula weight, 264.32 ug=1 micromole. 660.8ug=2.5 micromoles, 1.3216mg=5 micromoles.

if you want concentration then you add those amounts to 1 liter (final volume).

[deep]

mdfenko, on May 19 2010, 06:12 AM, said:

do you want concentration (micromolar) or amount (micromoles)?

based on the formula weight, 264.32 ug=1 micromole. 660.8ug=2.5 micromoles, 1.3216mg=5 micromoles.

if you want concentration then you add those amounts to 1 liter (final volume).

thanks for reply.
I need micromolar (μM) concentration of a drug.

[deep]

than4, on May 18 2010, 05:50 PM, said:

deep, on May 19 2010, 01:13 AM, said:

Can anybody tell me how to obtain micromolar concentration of any drug.
I want to test 2.5 and 5 micromoles of drug in cell culture. molecular weight of the drug is 264.32.

I would made up a millimolar stock (say 1mM) and dilute appropriately to get micromolar concentrations in your media.

e.g. for a 1uM concentration in 1mL of media add 1uL of 1mM stock.

Thanks for help.
Can you please tell me at which step, mostly error occurs during MTT assay?
I am doing for the first time.
thanks.