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how to prepare micromolar drug concentration


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#1 deep

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Posted 18 May 2010 - 05:13 PM

Can anybody tell me how to obtain micromolar concentration of any drug.
I want to test 2.5 and 5 micromoles of drug in cell culture. molecular weight of the drug is 264.32.

#2 than4

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Posted 18 May 2010 - 05:50 PM

Can anybody tell me how to obtain micromolar concentration of any drug.
I want to test 2.5 and 5 micromoles of drug in cell culture. molecular weight of the drug is 264.32.


I would made up a millimolar stock (say 1mM) and dilute appropriately to get micromolar concentrations in your media.

e.g. for a 1uM concentration in 1mL of media add 1uL of 1mM stock.

#3 mdfenko

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Posted 19 May 2010 - 06:12 AM

do you want concentration (micromolar) or amount (micromoles)?

based on the formula weight, 264.32 ug=1 micromole. 660.8ug=2.5 micromoles, 1.3216mg=5 micromoles.

if you want concentration then you add those amounts to 1 liter (final volume).
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#4 deep

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Posted 20 May 2010 - 09:01 PM

do you want concentration (micromolar) or amount (micromoles)?

based on the formula weight, 264.32 ug=1 micromole. 660.8ug=2.5 micromoles, 1.3216mg=5 micromoles.

if you want concentration then you add those amounts to 1 liter (final volume).


thanks for reply.
I need micromolar (μM) concentration of a drug.

#5 deep

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Posted 20 May 2010 - 09:05 PM

Can anybody tell me how to obtain micromolar concentration of any drug.
I want to test 2.5 and 5 micromoles of drug in cell culture. molecular weight of the drug is 264.32.


I would made up a millimolar stock (say 1mM) and dilute appropriately to get micromolar concentrations in your media.

e.g. for a 1uM concentration in 1mL of media add 1uL of 1mM stock.



Thanks for help.
Can you please tell me at which step, mostly error occurs during MTT assay?
I am doing for the first time.
thanks.




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