Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Fibronectin coating


  • Please log in to reply
1 reply to this topic

#1 Irene

Irene

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 18 May 2010 - 06:15 AM

Hi, I'm gonna coat my plates with fibronectin and first of all I want to know if what I've plan to do is ok:

Coat plates with 10g/ml fibronectin in PBS1x. Incubate ON at 4C.

Rinse plates once with PBS 1x.

Pre-warm plates at 37C before seeding.

And secondly, I wonder if I can reuse fibronectin solution used for coating and if so, how to keep it.

Thanks

#2 Acehunter

Acehunter

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 22 November 2010 - 09:48 AM

Hi.

What we generally do, is to add 1mL of a 10g/mL Fibronectin solution (fibronectin diluted in PBS 1x pH7.4) to each well, and then incubate at 37C 5% CO2 for 1h. Then we rinse the plates with PBS (1x pH7.4), before adding the cells. This cuts the time you need for cell culture preparations by a lot.

Ace




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.