Stability of genomic DNA in 1x PBS
Posted 18 May 2010 - 04:44 AM
I perform heat-induced genomic DNA extraction from bacterial cells, and at the end they are stored in 4C in 1x PBS. I would like to know if there is any data/paper showing the duration in which they are stable for.
This article from QIAGEN shows that using their Buffer AE purified DNA is stable for up to 8 years in 2-8C. However, of course, the chemical makeup of AE is different from PBS.
Posted 18 May 2010 - 12:09 PM
Posted 18 May 2010 - 12:40 PM
730 What is the composition of buffer AE?
The composition of Buffer AE is:
10 mM Tris-Cl
0.5 mM EDTA; pH 9.0.
Buffer AE is the elution buffer used in DNeasy Kits for genomic DNA isolation from animal and plant samples, and QIAamp Kits for DNA isolation from clinical samples.