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primer design by generunner DG


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#1 sara.r

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Posted 18 May 2010 - 03:36 AM

hi
I have a question about primer design by generunner :
is there any accepted treshould of dG in analysis of hairpin or dimers?

regards

#2 criscastells

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Posted 18 May 2010 - 08:03 AM

I had this same problem some days ago. I read a lot of bibliography and I use these tresholds:
dG>-3Kcal/mol for hairpins and dG>-6Kcal/mol for dimers.

I read that larger negative valuer for dG indicate stable, undesirable structures.

I hope this information will be helpful for you




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