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Murine Cell Lines


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#1 yoshi

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Posted 17 May 2010 - 08:42 PM

Dear all, is there a web site that shows if a protein is expressed in a certain murine cell line?

I need to know in which murine cell lines are mFlt3 Ligand and mSmad8 constitutively expressed. They need not be the same cell line.

Thanks in advance!

#2 lab rat

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Posted 17 May 2010 - 10:14 PM

Can you transfect the cells to express these proteins?

also, Miltenyi has a purified Flt-3 ligand available.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 yoshi

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Posted 17 May 2010 - 11:06 PM

Can you transfect the cells to express these proteins?

also, Miltenyi has a purified Flt-3 ligand available.


I have already done that, but I couldn't detect the protein with a newly purchased antibody. So I need a positive control cell line to check if it's the antibody or my DNA construct.

Thanks anyway.




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