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Two peaks measured by DCFH-DA


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#1 sndrlai

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Posted 15 May 2010 - 10:15 PM

Hi,
I have tried using DCFH-DA in assessing the ROS generation in my cells (Caco-2). It is supposed that with my sample added, there should be less ROS generated. However, after I did the flow cytometry, I found out that there were two peaks in the histogram of the control and only one peak was shifted (but not the other) in the cells with sample added.
Does anyone know the reason behind?
Is it the interactions between my cells and samples are not good enough so that only a portion of cells can respond to my sample?
And can I conclude that my sample is effective in decreasing the ROS generation in Caco-2 based on this result? Or shifts in both peaks are necessary in getting a conclusion?
Please help me.

#2 jay102

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Posted 22 June 2010 - 06:18 PM

The cytochrome c complex is not the only electron transport chain involved in respiration -- see, for example, the Krebs cycle (aka the citric acid cycle).

I've met this problem but I don't remember the solution!




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