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#1 vered

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Posted 15 May 2010 - 02:56 PM

i need to make a mouse that has a tag on my desired protein. The application will be for immuno-precipitation and immuno- fluorescence (FISH) mostly. Does anyone have a knowledge about this subject, like which tag (FLAG, HA, MYC, GFP), if it better on the N or C terminus, shold i fuse with it a linker of glycine for example or fuse straight to my protein?
any information will be helpful
thank you very much
V

#2 lab rat

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    Why does a science forum not have pictures of mice and rats?

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Posted 18 May 2010 - 07:45 AM

I have not made transgenic animals myself, but I can direct you to a few papers.

These are links to some papers by Barmada and Ivanova that describe prion-GFP GMO mice. They discuss how they were made, how they determined the placement of the GFP tag, etc. That should get you started.

Good luck.

http://www.ncbi.nlm....pubmed/15262264
http://www.jneurosci...ract/25/24/5824
http://www.jneurosci...ract/25/24/5824
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 vered

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Posted 22 May 2010 - 04:02 PM

thank you very much
V

I have not made transgenic animals myself, but I can direct you to a few papers.

These are links to some papers by Barmada and Ivanova that describe prion-GFP GMO mice. They discuss how they were made, how they determined the placement of the GFP tag, etc. That should get you started.

Good luck.

http://www.ncbi.nlm....pubmed/15262264
http://www.jneurosci...ract/25/24/5824
http://www.jneurosci...ract/25/24/5824






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