Posted 15 May 2010 - 01:21 AM
Posted 15 May 2010 - 11:43 AM
Too much can kill them, so do titrations to determine the minimum amount required to kill the untransformed cells. If you use the minimum inhibitory concentration, you won't harm your cells but will keep contaminants at bay.
Posted 16 May 2010 - 04:20 PM
The use of antibiotics for routine culture masks cryptic infections that will otherwise become apparent very quickly. With good technique you should not have to use antibiotics at all (apart from keeping the plasmids in stably transfected cells).