2 replies to this topic

[nocas]

Hi!

I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.

Thanks

[Prep!]

AnaG, on May 15 2010, 01:56 AM, said:

Hi!

I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.

Thanks

well we always use a fresh one wen needed so not sure about the stability but i wud suggest if u not running short of the components prepare fresh.. alternatively try to smell if the mercapto is still tere or gone!!!

[mdfenko]

two weeks at room temperature is too long for mercaptoethanol.

make fresh.