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stability of the stripping buffer


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#1 nocas

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Posted 14 May 2010 - 11:26 AM

Hi!

I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.

Thanks

#2 Prep!

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Posted 14 May 2010 - 07:31 PM

Hi!

I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.

Thanks


well we always use a fresh one wen needed so not sure about the stability but i wud suggest if u not running short of the components prepare fresh.. alternatively try to smell if the mercapto is still tere or gone!!!
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#3 mdfenko

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Posted 18 May 2010 - 11:09 AM

two weeks at room temperature is too long for mercaptoethanol.

make fresh.
talent does what it can
genius does what it must
i do what i get paid to do




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