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radiolabeled protein uptake


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#1 AnnaSh

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Posted 14 May 2010 - 07:36 AM

Hi all!
I have to study the uptake process of a radiolabeled protein in adherent cell cultures, but I have no experience in the field. Can anyone give me some practical suggestions or suggest a basic protocol?

Thank you in advance for help!

#2 bob1

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Posted 16 May 2010 - 04:18 PM

The very first thing you should do is read the local regulations on storage, handling and disposal of radioactive substances. You will probably find that you need some sort of dedicated room/area, equipment and a radiation license holder to enable you to do this sort of work.

Your best bet is to find someone experienced to sit alongside so that you can learn by example. There are all sorts of little things that can make your radioactive work run much more smoothly with less exposure to radiation and less risk of contaminating lots of stuff.

#3 AnnaSh

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Posted 18 May 2010 - 01:33 AM

Thank you very much for your advices!

We have special rooms dedicated to radioactivity and our technician can give me indications for working safe. I was searching for a protocol to correctly perform a test, for example you may need to detach cells from plates with trypsin or they can be dissolved by a particular solvent mixture?
What kind of tubes I have to use?...and so on.

I hope you can help me, thank you.




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