Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Roche DIG in situ hybridization system

  • Please log in to reply
2 replies to this topic

#1 cathywill



  • Active Members
  • Pip
  • 8 posts

Posted 14 May 2010 - 06:59 AM

I am new to ISH. I found this non-radioactive system in Roche. Has anybody used this before? Is it good? I am gonna do this on tissue slide. Is it possible to use the same slide for ISH, and then immunohistochemistry?
I appreciate your suggestions.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,130 posts

Posted 16 May 2010 - 04:09 PM

The system is definitely good, there are a range of ways of generating probes and the detection is easy and fast compared to radioactive systems. I don't know if you could subsequently do IHC on them as I guess will probably destroy the DIG label, but it may be OK, depending on the system you are using.

#3 Rsm


    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 385 posts

Posted 16 May 2010 - 11:26 PM

I have used the Roche System extensively, and confirm that it's pretty good once you got rid of your background. We used anti-DIG HRP and Cy3 TSA (Perkin Elmer) for ISH and antibody staining to stain cells of interest. It took some time to optimize, but we got a good signal/noise ratio eventually... Please check the pic.



Attached Thumbnails

  • Dct_long.jpg

I got soul, but I'm not a soldier

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.