4 replies to this topic

[moljul]

currently i am busy doing searching the "right" cell lines for my experiements!
i am not that familiar with colorectal, mammary gland, prostate and pancreas carcinoma cells!

i am in general interested why some cell lines got so "popular" - why are they the bestsellers and published so often!!

colorectal: CaCo-2, HCT116...
prostate: DU145, LNCap, PC3...
mammary gland: MCF-7, MDA-MB-231...
pancreas: Panc-1, MIA PaCa-2...

ths in advance

[bob1]

Easy to grow is a big player, also some are better characterised than others, some have particular characteristics that are representative of a particular cell type or express genes in a more "normal" fashion.

[tea-test]

in breast cancer cells , for instance, you can differentiate between hormone dependent (-> MCF-7) and hormone-independent (->MDA-MB-231) cell lines.

or if you want to have a model for an epithelial tumor you should not choose a cell line that was established from a stromal tumor. that's what common sense is telling me  

[arera]

in breast cancer cells , for instance, you can differentiate between hormone dependent (-> MCF-7) and hormone-independent (->MDA-MB-231) cell lines


Good comment tea test!!!  

[Inmost sun]

moljul, on May 14 2010, 01:44 PM, said:

currently i am busy doing searching the "right" cell lines for my experiements!
i am not that familiar with colorectal, mammary gland, prostate and pancreas carcinoma cells!

i am in general interested why some cell lines got so "popular" - why are they the bestsellers and published so often!!

colorectal: CaCo-2, HCT116...
prostate: DU145, LNCap, PC3...
mammary gland: MCF-7, MDA-MB-231...
pancreas: Panc-1, MIA PaCa-2...

ths in advance

I think it is an evolutionary process; one or some start with a cell line and establish handling, biological parameters and scientic questions ("hormone dependency" was argued); so it is more easy to refer to previous works than establishing a novel cell line