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House keeping gene question?


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#1 huzazhang

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Posted 14 May 2010 - 03:39 AM

Hey:

I am performing some RTPCR rns, my gene of interest have a ct vale of around 25-26 and my house keeping genes hALAS and Beta actin, have Ct values of around 23 and 17 respectively. Which one would be better to use as a house keeper. The Halas has ct values closer to my gene interest and also shows the result I want whereas beta actin do not.

Thanks for the help!

HUz

#2 ivanbio

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Posted 14 May 2010 - 06:22 AM

The closer the Ct values between your gene of interest and your housekeeping genes, the better. Plus, nALAS is giving you the results you are looking for, so I would go with that one and forget about beta actin.

Having said this, it is recommended to always have at least 2 or 3 housekeeping genes give you similar results in order to believe that what you are getting is true. I would discard beta actin but look for at least one more housekeeping gene.

Good luck

Ivan
Carlsbad, CA




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