Hey:
I am performing some RTPCR rns, my gene of interest have a ct vale of around 25-26 and my house keeping genes hALAS and Beta actin, have Ct values of around 23 and 17 respectively. Which one would be better to use as a house keeper. The Halas has ct values closer to my gene interest and also shows the result I want whereas beta actin do not.
Thanks for the help!
HUz
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ivanbio
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Posted 14 May 2010 - 06:22 AM
The closer the Ct values between your gene of interest and your housekeeping genes, the better. Plus, nALAS is giving you the results you are looking for, so I would go with that one and forget about beta actin.
Having said this, it is recommended to always have at least 2 or 3 housekeeping genes give you similar results in order to believe that what you are getting is true. I would discard beta actin but look for at least one more housekeeping gene.
Good luck
Having said this, it is recommended to always have at least 2 or 3 housekeeping genes give you similar results in order to believe that what you are getting is true. I would discard beta actin but look for at least one more housekeeping gene.
Good luck
Ivan
Carlsbad, CA
