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Concentrating RNA


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#1 Lakia

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Posted 13 May 2010 - 10:14 PM

Hi all,

I have a few samples of RNA that I want to use for RACE PCR. The concentration in the samples is too low (around 30-60ng/microlitre) to make RACE cDNA at the moment but I think I can get a high enough concentration if I pool my samples (I have about 30 microlitres from several RNA extractions) and concentrate them down to about 8 microlitres. I am just wondering what the best method for concentrating the RNA. We have a speed vac in the building, but I think it only runs at ambient temperature at the best so I am a bit worried about degrading the RNA. Ive also considered ethanol precipitation but I'm worried about losing some RNA through that method. Ive had another suggestion that I can leave the sample in a fume hood and just let the water evaporate but that sounds a bit risky also. What is the safest method to concentrate the RNA?

#2 epibio

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Posted 14 May 2010 - 11:53 AM

As long as you don't mind losing some of the very small RNA, Zymo's RNA Clean and Concentrator columns work pretty well.

#3 Prep!

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Posted 14 May 2010 - 07:33 PM

As long as you don't mind losing some of the very small RNA, Zymo's RNA Clean and Concentrator columns work pretty well.


i guess u can use any valid cut off centrifuge filters from any of the vendors... that way even the temperature u can control!!!
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#4 moerae

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Posted 18 May 2010 - 07:30 PM

We normally just precipitate the RNA out of the solution using a carrier and resuspend in whatever volume we want.




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