Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Quantity of Protein from fractionation

  • Please log in to reply
No replies to this topic

#1 jaknight



  • Active Members
  • Pip
  • 13 posts

Posted 13 May 2010 - 02:37 PM

I am looking into doing a plasma membrane fractionation using a sucrose gradient. I was wondering if anyone has any experience with this and could let me know how much protein you were able to extract.

I will be working with cells and am trying to estimate the amount of membrane protein I can get from a 10cm plate. I know the amount will be dependent upon the cell type, culture conditions and fractionation procedure, but I am just looking to get a rough idea of what to expect from this. Thanks.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.