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NK cell staining


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4 replies to this topic

#1 Andrew

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Posted 13 May 2010 - 12:53 PM

i'm going to start some nk experiments soon and i did a few test stains of splenocytes. nk cells should be nk1.1 nkg2d double positive but i only seem to see single positives. anyone have advice?

#2 leelee

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Posted 16 May 2010 - 07:36 PM

What mouse strain are your NK cells from?

#3 Andrew

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Posted 26 May 2010 - 11:35 AM

What mouse strain are your NK cells from?

balb/c

#4 leelee

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Posted 26 May 2010 - 06:23 PM

There is your problem- Balb/c mice don't express NK1.1!!!

#5 ribonucleic

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Posted 19 August 2013 - 05:07 AM

Actually, several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (a pan NK-antibody that recognizes CD49b).

 

http://www.ebioscien...ified-pk136.htm






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