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MTT assay


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#1 Penguin

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Posted 13 May 2010 - 10:36 AM

Hey!

Does anyone know why all the MTT assay protocols tell you to subtract the background at 630 nm when you take the readings at 570 nm???

Thanks,
P

#2 bob1

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Posted 13 May 2010 - 04:48 PM

It will be that the MTT absorbs most strongly at 570 nm, but there are other things which interfere with the readings which can also be measured at 630 nm, so you can subtract the 630 nm value and work out what is actual signal.

#3 Penguin

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Posted 13 May 2010 - 10:09 PM

Oh ok, I guess cell debris and serum etc.

Thanks bob1!




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