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Precipitation of protein


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#1 samita

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Posted 12 May 2010 - 01:19 AM

I need to precipitate my protein before loading to SP column with amoinum sulphate 70 % saturation...
can some body tell me how to 70% saturate the supernatent with amonium sulphate?????
regards

#2 Prep!

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Posted 12 May 2010 - 01:45 AM

I need to precipitate my protein before loading to SP column with amoinum sulphate 70 % saturation...
can some body tell me how to 70% saturate the supernatent with amonium sulphate?????
regards


well i ve never done it but may be this might help you!!

http://www.encorbio....cols/AM-SO4.htm
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#3 samita

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Posted 12 May 2010 - 01:46 PM

Thanks I will try

I need to precipitate my protein before loading to SP column with amoinum sulphate 70 % saturation...
can some body tell me how to 70% saturate the supernatent with amonium sulphate?????
regards


well i ve never done it but may be this might help you!!

http://www.encorbio....cols/AM-SO4.htm



#4 swanny

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Posted 16 May 2010 - 05:54 PM

Are you working from a paper? If so, you might want to do some trials at lower NH4.SO4 concentrations, in order to get rid of other proteins that precipitate below 70%, unless, of course, your protein is still soluble at 70% and you're getting rid of everything else...

Edited by swanny, 16 May 2010 - 05:55 PM.

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