3 replies to this topic

[samita]

I need to precipitate my protein before loading to SP column with amoinum sulphate 70 % saturation...
can some body tell me how to 70% saturate the supernatent with amonium sulphate?????
regards

[Prep!]

samita, on May 12 2010, 02:49 PM, said:

I need to precipitate my protein before loading to SP column with amoinum sulphate 70 % saturation...
can some body tell me how to 70% saturate the supernatent with amonium sulphate?????
regards

well i ve never done it but may be this might help you!!

http://www.encorbio....cols/AM-SO4.htm

[samita]

Thanks I will try

Prep!, on May 12 2010, 01:45 AM, said:

samita, on May 12 2010, 02:49 PM, said:

I need to precipitate my protein before loading to SP column with amoinum sulphate 70 % saturation...
can some body tell me how to 70% saturate the supernatent with amonium sulphate?????
regards

well i ve never done it but may be this might help you!!

http://www.encorbio....cols/AM-SO4.htm

[swanny]

Are you working from a paper? If so, you might want to do some trials at lower NH4.SO4 concentrations, in order to get rid of other proteins that precipitate below 70%, unless, of course, your protein is still soluble at 70% and you're getting rid of everything else...

Edited by swanny, 16 May 2010 - 05:55 PM.