Can anyone advise me of the best transfection reagent to use for cotransfecting siRNA and plasmid in HeLa cells?I tried lipofectamine200 and it doesnt seem to be working.Thanks I appreciate it !
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Cotransfection of siRNA and plasmid
Started by Julie123, May 11 2010 05:53 PM
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#1
Julie123
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Posted 11 May 2010 - 05:53 PM
Hello
Can anyone advise me of the best transfection reagent to use for cotransfecting siRNA and plasmid in HeLa cells?I tried lipofectamine200 and it doesnt seem to be working.Thanks I appreciate it !
Can anyone advise me of the best transfection reagent to use for cotransfecting siRNA and plasmid in HeLa cells?I tried lipofectamine200 and it doesnt seem to be working.Thanks I appreciate it !
#2
pcrman
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Posted 21 May 2010 - 08:01 PM
Cotransfecting plasmid and siRNA using lipofectamine 2k should not be a problem. What problem do you have: low efficiency of siRNA or plasmid transfection? Probably you need to optimize the relative concentration of each component. If it still does not work, try sequential transfection.
