Can anyone advise me of the best transfection reagent to use for cotransfecting siRNA and plasmid in HeLa cells?I tried lipofectamine200 and it doesnt seem to be working.Thanks I appreciate it !
Submit your paper to J Biol Methods today!
Cotransfection of siRNA and plasmid
Started by Julie123, May 11 2010 05:53 PM
1 reply to this topic
#1
Julie123
-
- Active Members
-
- 11 posts
member
0
Neutral
Neutral
Posted 11 May 2010 - 05:53 PM
Hello
Can anyone advise me of the best transfection reagent to use for cotransfecting siRNA and plasmid in HeLa cells?I tried lipofectamine200 and it doesnt seem to be working.Thanks I appreciate it !
Can anyone advise me of the best transfection reagent to use for cotransfecting siRNA and plasmid in HeLa cells?I tried lipofectamine200 and it doesnt seem to be working.Thanks I appreciate it !
#2
pcrman
-
- Global Moderators
-
- 1,142 posts
Epigenetist
63
Excellent
Excellent
Posted 21 May 2010 - 08:01 PM
Cotransfecting plasmid and siRNA using lipofectamine 2k should not be a problem. What problem do you have: low efficiency of siRNA or plasmid transfection? Probably you need to optimize the relative concentration of each component. If it still does not work, try sequential transfection.
