4 replies to this topic

[PandaCreamPuff]

Hi all

I would like to know if formamide is preferably used during sequence analysis. If so why is this the case?

Thanks :-D

[mdfenko]

formamide is preferred (mandatory) when using beckman-coulter sequencers.

[PandaCreamPuff]

ah. We have an AB 3130 Analyser and, based on convention of our lab, we use formamide as well  

Just wondering if there is any biochemical basis supporting its use in optimising sequencing results.

[mdfenko]

the wellred dyes used by beckman-coulter decompose at low pH (<6). water will absorb atmospheric carbon dioxide and the pH will drop. formamide, as long as it is deionized and doesn't decompose (keep water out of it, avoid freeze-thaw cycles), will not acidify as water will so the dyes will be more stable.

[PandaCreamPuff]

ah thanks.