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help preparing broth suspension


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4 replies to this topic

#1 maryjo

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Posted 11 May 2010 - 12:02 AM

I have never used a nutrient broth (always agar), but i need to use it now. My question is how do i inoculate nutrient broth for incubation...i know i have to make a bacterial suspension in saline first, but how much suspension do i put in how much broth (i want to try to keep every preperation at a similar concentration 0.5 Mcfarland for example).

#2 PandaCreamPuff

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Posted 11 May 2010 - 05:28 AM

someone correct me if wrong, but depending on the bacterium and volume of your broth, do you not necessarily need to make a growth culture. Instead you can just use colonies from plates and inoculate a colony into the broth. I tend to keep the inoculator inside a 10ml culture in falcon tube after swabbing a colony from blood agar.

#3 pito

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Posted 13 May 2010 - 08:16 AM

you can simply take a colony from your plate and then inoculate the broth with that.
Just use a inoculating loop or needle for it.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#4 ProteinWork

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Posted 13 May 2010 - 08:11 PM

you can simply take a colony from your plate and then inoculate the broth with that.
Just use a inoculating loop or needle for it.

Or a sterile pipette tip. Anything will do as long as it's sterile.

#5 maryjo

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Posted 16 May 2010 - 11:21 AM

Thanks alot!




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