Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Measuring DNA, RNA, protein and cell wall synthesis


  • Please log in to reply
1 reply to this topic

#1 gltm

gltm

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 10 May 2010 - 05:03 AM

Hi!

I need to measure DNA, RNA, protein and cell wall synthesis in G- & G+ bacteria after treatment with an anti-bacterial. Most of the protocols I've found involve measuring the incorporation of tritiated precursors. We aren't set up to work with radiation here - does anyone know any alternative methods?

Thanks!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,722 posts
399
Excellent

Posted 10 May 2010 - 04:26 PM

I haven't done these types of experiment, but have a look for pulse-chase experimentals, there may be some new methods out there in recent times.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.