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Making stable cell line expressing large gene


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#1 kingsupercat

kingsupercat

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Posted 10 May 2010 - 02:19 AM

Hi,

Could anyone give me some advises on how to make permanent cell line expressing large gene (about 2,088 amino acids)?
I'd tried for many times, but the element seemed to be difficult to integrate into cells' genome...
Thank you very much.




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