Hi, I am using Annexin V and propodium iodide to test the apoptosis in THP-1 cells.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.
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Posted 14 May 2010 - 05:01 AM
goldfinger, on May 7 2010, 07:09 PM, said:
Hi, I am using Annexin V and propodium iodide to test the apoptosis in THP-1 cells.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.
PI positive cells and annexin V negative cells are supposed to be necrotic cells (unless you damaged the membranes of these cells during preparation of the samples).
