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Propodium Iodide postive and Annexin V negative


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#1 goldfinger

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Posted 07 May 2010 - 09:09 AM

Hi, I am using Annexin V and propodium iodide to test the apoptosis in THP-1 cells.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.

#2 theo22

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Posted 14 May 2010 - 05:01 AM

Hi, I am using Annexin V and propodium iodide to test the apoptosis in THP-1 cells.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.

PI positive cells and annexin V negative cells are supposed to be necrotic cells (unless you damaged the membranes of these cells during preparation of the samples).




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