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What is the pricinple behind 1.8g NaHCO3 per litter when CO2 is 5%?


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#1 AllenChiu

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Posted 07 May 2010 - 05:09 AM

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?

#2 Inmost sun

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Posted 09 May 2010 - 12:54 PM

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?


NaHCO3 + H2O = Na+ + H2CO3 + OH- = CO2 + H2O + Na+ + OH-

or

NaHCO3 + 2H2O +CO2 = Na+ + 2H2CO3 +OH- = 2CO2 + 2H2O +Na+ + OH-

NaHCO3 and CO2 will be in balance, regard the K for NaHCO3, and K for H2CO3; to calculate the correct percentage of CO2 you also need the incubator volume

#3 AllenChiu

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Posted 10 May 2010 - 07:58 AM

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?


NaHCO3 + H2O = Na+ + H2CO3 + OH- = CO2 + H2O + Na+ + OH-

or

NaHCO3 + 2H2O +CO2 = Na+ + 2H2CO3 +OH- = 2CO2 + 2H2O +Na+ + OH-

NaHCO3 and CO2 will be in balance, regard the K for NaHCO3, and K for H2CO3; to calculate the correct percentage of CO2 you also need the incubator volume

great,Thank you!

#4 hobglobin

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Posted 10 May 2010 - 08:23 AM

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?


NaHCO3 + H2O = Na+ + H2CO3 + OH- = CO2 + H2O + Na+ + OH-

or

NaHCO3 + 2H2O +CO2 = Na+ + 2H2CO3 +OH- = 2CO2 + 2H2O +Na+ + OH-

NaHCO3 and CO2 will be in balance, regard the K for NaHCO3, and K for H2CO3; to calculate the correct percentage of CO2 you also need the incubator volume


But temperature (and presumable pressure) should also be considered, as it influences the balance too a lot...i.e. the specification should be completed with a fixed temperature such as 20C, or STP.

Edited by hobglobin, 10 May 2010 - 08:57 AM.

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...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

That is....if she posts at all.





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