2 antibodies crossreacting with another protein
Posted 07 May 2010 - 03:58 AM
Can ANYONE help me!
I've generated 2 affinity purified antibodies against my protein of interest and BOTH cross-react with another protein.
The antigenic peptides used to generate these antibodies are seperate.
They were injected into the same rabbits but independently affinity purified.
There is no similarity in either antigenic peptide sequence with this other protein.
I cannot think of a reason why two independently affinity purified antibodies would cross-react with the same protein. Does anyone have any suggestions? As I can't explain it!
Posted 07 May 2010 - 05:12 AM
If this is polyclonal sera, perhaps you could adsorb out the cross-reacting population?
Posted 10 May 2010 - 07:44 AM