4 replies to this topic

[dee.]

hi!

does anyone know how it would be possible to fixate bacterial spores (B. subtilis) with all the required nutrients into a microtitre plate, so that when a reagent (liquid) is added the nutrients dissolve and the spores begin to germinate?
which substance could be used to fix it all together?

thanx!

[pito]

why cant you just put the spores in the plate and then add the medium ?

I do not see the point or your goal.

You want to preserve spores but not germinate them in a 96 well plate and later on when needed you want to germinate them? Is this what you mean?


or?

[HomeBrew]

I agree with pito -- why can't the "liquid reagent" that you add be media?

[dee.]

yes that is what i mean... it is all only for an imaginary bioassay, and i don't know if it is possible to fix spores or spores+nutrients to the plate?

pito, on May 7 2010, 01:52 PM, said:

why cant you just put the spores in the plate and then add the medium ?

I do not see the point or your goal.

You want to preserve spores but not germinate them in a 96 well plate and later on when needed you want to germinate them? Is this what you mean?


or?

[pito]

dee., on May 7 2010, 04:58 PM, said:

yes that is what i mean... it is all only for an imaginary bioassay, and i don't know if it is possible to fix spores or spores+nutrients to the plate?

pito, on May 7 2010, 01:52 PM, said:

why cant you just put the spores in the plate and then add the medium ?

I do not see the point or your goal.

You want to preserve spores but not germinate them in a 96 well plate and later on when needed you want to germinate them? Is this what you mean?


or?

Offcourse you can add spores in a 96well plate and keep them there.
However I do not completely understand what you want or what your goal is, its a strange task.
But it is possible to store spores with or without nutrients in such plates.