I just began a project about secreted miRNAs in non small-cell lung cancer.
i d like to know if anyone have already done such miRNA isolation from large amount of culture supernatant and if yes how !
I have also heard about an new isolation kit for exosomes generaly. and because those miRNa are retained in exosomes, it might be interesting to use this.
it s called ExoQuick from System Bioscience.
here is the link : http://www.systembio...quick-exosomes/
It is kind of weird because they manage to precipitate exosome with one reagent, in one step and only with a slow centrifugation !
the protocol I got implies a total 7 hrs of centrifugation steps and at realy high speed !
thanks for your advices and thoughts !
Submit your paper to J Biol Methods today!
Secreted miRNA isolation (from exosomes)
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