but when i run in the presence of the original plasmid sample which i made transfomation from it ,
i found a difference ,
all samples are not digested, the plasmid is as it is.
first lane is original plasmid (isolated from more than year)
second and fourth lane are the same plasmid isolated from jm109
third lane is the same plasmid but extracted from xl blue strain(transformed in another day)
all must be CMV-pbk
note: when i subculture in a liquid medium for plasmid isolation , i did not add the antibiotic.
So i am very worried.
how can i be sure from my result???
and can anybody describe for me this picture?
Edited by aidaelshaer, 06 May 2010 - 03:41 PM.